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Apolipoproteins are the protein components of lipoproteins in plasma and determine the structure, function, and metabolism of lipoproteins. Apolipoprotein A-I and A-II constitute about 90% of the total HDL protein. The ratio of Apo A-I to Apo A-II in HDL is about 3:1. Apo A-I and Apo A-II modulate HDL metabolism. Apo A-I and Apo A-II also function as cofactors of LCAT in the conversion of free cholesterol to cholesterol esters.
In this procedure, the HDL fraction is obtained via precipitation (see HDL-C precipitation by DS or PEG). Apo A-II is then measured on the HDL supernate by automated immunoturbidimetric assay.
* Please contact nexelis for information on stability.