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Apolipoproteins are components of lipoproteins in plasma and are important in determining the structure, function and metabolism of lipoproteins. Apolipoprotein C-II is contained in very low density lipoproteins (VLDL) and high density lipoproteins (HDL), and activates lipoprotein lipase (LPL) on the surface of endothelial cells. In type II dyslipidemia patients a strong positive correlation between plasma TG and apo C-II and C-III concentrations exists. However, the most pronounced increase in plasma apolipoproteins is observed in type V dyslipidemia patients. In these patients, plasma TG are positively correlated with apo C-III and inversely correlated with apo C-II.
In this procedure, the HDL fraction is obtained via precipitation (see HDL-C precipitation by DS or PEG). Apo C-II is then measured on the HDL supernate by automated immunoturbidimetric assay.