Neomed Labs Pacific Biomarkers PAIRimmune are now Nexelis
 

Cellular Immunology

Nexelis has a vast experience in developing cellular immunology assays to monitor immune cells during preclinical studies or clinical trials for the assessment of immunogenicity or efficacy of candidates from early to late-stage development.

Our experts can work in close collaboration with clients to adapt, develop, qualify and validate specific assays to appropriately assess cell-mediated immune responses and phenotyping.

Flow Cytometry

Flow cytometry provides detailed information on immune cell populations by detecting the expression of specific cell markers. This technology is also used for cytokine staining (intracellular or circulating), to determine impact on signaling pathways, apoptosis, phagocytic and oxidative burst activity and to measure cell proliferation and viability.

Various flow cytometers are available: Fortessa X20, BD FACS LSRII, & BD FACS Canto II.

Applications

  • PBMC isolation and cryopreservation
  • Immunophenotyping (up to 18 colors)
  • Intracellular cytokine staining (ICS)
    • Cytokines (e.g. IFN-γ, IL-2, IL-13, IL-17, TNF-α)
    • Cytotoxic potential
      (e.g. CD107a, Granzymes, Perforin)
    • B-cell help (e.g. CD40L)
    • Memory profile (e.g. CD45RA, CCR7, CD27, CD28)
  • T-cell activation/stimulation (e.g. CD44, CD62L)
  • Immune checkpoints (e.g. PD-1, LAG-3, Tim-3)
  • Antibody Dependent Cell-mediated Cytoxicity (ADCC)
  • Cell Beads Array (CBA)
  • Proliferation and viability (e.g. CFSE, Ki67, Annexin)
  • Infiltrating immune cells

ELISpot (AID) (ImmunoSpot/FluoroSpot)

The Enzyme-Linked ImmunoSpot (ELISpot) assay evaluates the response of specific immune cells to a certain stimulus by measuring both the quantitative frequency of positive-responding cells, as well as the qualitative characterization of secretion of specific cytokines or other secreted immune molecules by those responding cells. The ELISpot assay is used to monitor the effect that immunotherapies have on antigen-specific immunity of B- and T-cells, as well as the effect on the Th1/Th2 balance.

Detection can be performed with either bright field or fluorescence.

Applications

  • PBMC isolation and cryopreservation
  • Humoral response (B-cell)
    • Detection of Total and Specific Antibody Secreting Cells (ASC) – IgG / IgA
    • Detection of memory B-cells
  • Cell-mediated immune response (T-cell)
    • Cytokines: Vaccine efficacy (IFN-γ), Quality immune response (Th1/Th2)
    • Perforin & Granzyme B
    • Cytotoxic T-cell response

back to top