Biological or Clinical Significance:
Glucagon-like peptide 1 (GLP-1) is a 3.5 kD incretin hormone secreted primarily from the enteroglucagon-producing L-cells in the small intestine. The main actions of GLP-1 are stimulation of insulin secretion, reduction of gastric emptying and inhibition of glucagon secretion. GLP-1 also appears to be a physiological regulator of appetite and food intake. Decreased secretion of GLP-1 may contribute to the development of obesity, and exaggerated secretion could be responsible for postprandial reactive hypoglycemia.GLP-1 (7-36) amide and GLP-1 (7-37) are the biologically active forms of GLP-1. In vivo, the active forms are rapidly degraded by the dipeptidyl peptidase IV (DPP-IV), therefore blood collected for assays that quantitatively measure active forms of GLP-1 must be immediately preserved with DPP-IV inhibitors.
Principle of Test Method:
The GLP-1 active assay is an ultra-sensitive kit designed to quantify human active GLP-1 in human plasma, serum, and other biological media. This immunoassay employs electrochemilunimescent detection.