Biological or Clinical Significance:
Pre β1-HDL is a discoid-shaped HDL particle of approximately 67 kDa that migrates with pre-β electrophoretic mobility on agarose gels. It contains apolipoprotein A-I (Apo AI), phospholipids and unesterified cholesterol. Pre β1-HDL removes cholesterol from peripheral tissues through the reverse cholesterol transport (RCT). Depletion of Pre β1-HDL results in reduced in vitro cholesterol efflux from fibroblasts.
The concentration of Pre β1-HDL in plasma is a reflection of the rate of its formation (result of cholesterol efflux and lipolysis of triglyceride-rich lipoproteins) and remodeling (through the action of LCAT). Thus, the level of Pre β1-HDL could reflect the sum of both of these steps.
Principle of Test Method:
The Pre β1-HDL assay is a sandwich ELISA.