CXCL-10 (IP-10)

Analyte: CXCL-10 (IP-10)
Specimen Type: Serum
Optimum Volume: 0.5 mL
Reporting Units: pg/mL
Method: ELISA
2-8°C 5 days
-20°C TBD
-70°C TBD

Biological or Clinical Significance:

IP-10 (interferon-gamma inducible protein 10 kDa), also known as CXCL10, was originally indentified as an IFN-γ-inducible gene.  It is induced in a variety of cells in response to IFN-γ and LPS.  In contrast to other CXC chemokines, IP-10 has no chemotactic activity for neutrophils.  It is a pleiotropic molecule that appears to target activated T cells and monocytes.  IP-10 inhibits bone marrow colony formation and angiogenensis.  It can also stimulate NK and T cell migration, regulate T cell maturation and modulate adhesion molecule expression.

IP-10 expression has been associated with HIV infection.  It can contribute to the accumulation of activated T cells in the cerebrospinal fluid compartment in HIV-1 infected individuals.  The retroviral transactivator, HIV-1 Tat, is a potent inducer of IP-10 expression in astrocytes.

IP-10 expression has also been shown to be significantly elevated in astrocytes within the brains of Alzheimer’s disease patients.  Astrocytes expressing IP-10 are commonly associated with senile plaques.

Principle of Test Method:

The IP-10 immunoassay is a solid-phase ELISA designed to measure human IP-10 in cell culture supernates, serum, plasma and saliva.  This assay employs the quantitative sandwich enzyme immunoassay technique.