Biological or Clinical Significance:
Matrix metalloproteinases (MMPs) constitute a family of zinc and calcium dependent endopeptidases that function in the breakdown of extracellular matrix (ECM). They play an important role in many normal physiological processes such as embryonic development, morphogenesis, reproduction, and tissue remodeling. They also participate in many pathological processes such as arthritis, cancer, and cardiovascular disease.
MMP-1 (also referred to as interstitial collagenase, vertebrate collagenase, fibroblast collagenase, or collagenase I) is produced by fibroblasts, chondrocytes, macrophages, keratinocytes, endothelial cells, and osteoblasts. MMP-1 is normally secreted as a 52 kDa pro-enzyme. Activation of the pro-enzyme involves a proteolytic removal of the pro-domain. MMP-1 is implicated in a wide variety of biological processes where collagen degradation occurs. These include rheumatoid arthritis, osteoarthritis, periodontal disease, tumor invasion, angiogenesis, corneal ulceration, tissue remodeling, inflammatory bowel disease, atherosclerosis, aneurysm, and restenosis.
Principle of Test Method:
The MMP-1 assay is a solid-phase ELISA that employs the quantitative fluorimetric enzyme immunoassay principle.
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