Custom Protein Services for Drug Discovery and Life Sciences

All projects begin with the analysis of physico-chemical and biological properties of a target protein resulting in the development of tailored procedures for its extraction, purification, and characterization.

Molecular Biology

Our comprehensive services include molecular synthesis, cloning, and modification. To address your protein’s critical quality characteristics, we engage in rational design.

Rational Design for Critical Quality Attributes
Protein structure characterizationApply bioinformatics
Alleviate solubility and expression challengesEvaluate fusion partners
Improve protein homogeneityOptimize post-translational modifications (PTM)
Promote expressionHarmonize RNA and DNA elements
Increase stabilityModify protease-susceptible sites
Our molecular biology services include:
Gene synthesis & sequencing
Molecular cloning protocol
DNA plasmid production
Other molecular biology assays (QPCR, RT-PCR, RNA, etc.)

Protein Expression

We provide multiple expression systems to produce recombinant proteins. Our protein design and expression optimization services facilitate the generation of well-characterized products. Early on, we aim to ensure:

  • Your preclinical product will be ready for scale-up
  • Your reagents are well-suited for biological assays
  • We are generating and selecting stable cell lines
  • Your biologics process will be feasible at a commercial scale

Protein Purification

To purify your proteins, we design product-specific purification strategies to reach the desired purity and key parameters. We work on a wide range of purification methods or improve upon existing purification protocols, when necessary.

Protein Structure Characterization

Proper characterization of your protein’s biophysical properties mitigates risk. Orthogonal evaluation of key parameters, such as size in solution, folding, and sequence integrity ensures quality and consistency of delivered material.

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Analytical Methods

Our protein science services encompass a range of analytical methods utilizing multiple assays that can be used to characterize a broad range of proteins expressed and purified by various methods.

  • SDS-PAGE/densitometry

  • Circular dichroism (CD)
  • UV/Vis absorbance spectroscopy
  • Intrinsic fluorescence (IF)
  • Differential scanning fluorimetry (DSF)
  • Differential scanning calorimetry (DSC)
  • Transmission electron microscopy (TEM)
  • N-glycan analysis

  • Colorimetric
    (Bradford/Lowry/ BCA/ RCDC Assays)
  • Absorbance at 280nm

  • Western blot
  • Peptide mapping (LC-MS/MS)
  • Chromatography

  • Analytical ultracentrifugation
  • Dynamic light scattering

  • Protein binding affinity (FortéBio) (Octet® RED96 System)
  • Enzymatic/colorimetric assays

  • Residual proteins
  • Residual DNA (qPCR)
  • Endotoxins (LAL)