Biological or Clinical Significance:
Measurement of cholesterol is primarily used in assessing risk of heart disease and to aid in the diagnosis of the lipoprotein disorders.
Principle of Test Method:
This procedure involves a Trinder reaction in which the amount of cholesterol present in the specimen is quantified following enzymic hydrolysis of cholesterol esters.
This method may be used to assay total cholesterol in serum, plasma or in lipoprotein fractions obtained by preparative ultracentrifugation (e.g. cholesterol in the VLDL fraction obtained in the beta quantification procedure).
Cooper GR, Duncan PH, Hazlehurst JS, et al. Cholesterol, enzymic method. In: Faulkner WR, Meites S, eds. Selected Methods for the Small Clinical Laboratory, Washington DC: AACC, 1982;9:165-74.
Warnick GR, Benderson J, Albers JJ. Dextran Sulfate Mg2+ Precipitation Procedure for Quantification of High Density Lipoprotein Cholesterol. In: Cooper GR, ed. Selected Methods of Clinical Chemistry, Washington DC: AACC, 1983;10:91-9.
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U.S. Department of Health and Human Services. The Lipid Research Clinics Population Studies Data Book. Volume I, The Prevalence Study. July 1980.
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